Manual of Practical Biochemistry for Medical Students

ISBN: 9788173719349 | Year: 2014 | Paperback | Pages: 236 | Language : English

Book Size: 180 x 240 mm | Territorial Rights: World

Price: 390.00

This book is a practical companion in biochemistry to students of MBBS and other allied sciences, and for those engaged in biochemistry laboratories. Completely revised and updated, this new edition presents the subject in a lucid style and can be easily assimilated. The language is simple and easy to understand. The book contains relevant illustrations, tables and boxes, making it reader-friendly. The content is organised into 31 chapters of qualitative analyses and quantitative estimations, covering principles, procedures and observations followed by clinical interpretation. Specially formulated MCQs, problem-based exercises, questions relating to interpretation of laboratory data and case studies are provided to help students prepare for competitive examinations in India and abroad.

Rafi , MBBS MD FIMSA, is Professor of Biochemistry at Dr NTR University of Health Sciences, Andhra Pradesh.

Preface to the Second Edition

Preface to the First Edition


1. Safety in the Laboratory

2. Reactions of Carbohydrates

Reactions of Glucose

Reactions of Fructose

Reactions of Maltose

Reactions of Lactose

Reactions of Sucrose

Identification of Unknown Carbohydrate

3. Reactions of Lipids

Reactions of Triacylglycerols

Reactions of Cholesterol

4. Reactions of Proteins

Precipitation Reactions of Proteins

Colour Reactions of Proteins

Reactions of Albumin

Reactions of Casein

Reactions of Gelatin

Reactions of Peptone

Identification of Unknown Protein

5. Reactions of Non-protein Nitrogenous (NPN) Substances


Uric Acid


Identification of Unknown NPN Substance

6. Identification of Unknown Substance of Biochemical Importance

Carbohydrates, Proteins and NPN Substances

7. Analysis of Urine

Physical Examination

Microscopic Examination

Chemical Analysis

8. Analysis of Abnormal Constituents in Urine


Ketone Bodies



Bile Salts

Bile Pigments

Identification of Unknown Abnormal Constituents in Urine

9. Qualitative Analysis of Saliva

10. Qualitative Analysis of Gastric Juice

11. Qualitative Analysis of Bile

12. Qualitative Analysis of Milk

13. Food Analysis


14. Introduction to Clinical Biochemistry

Glassware and Instruments Used in the Laboratory


Sample Collection and Handling

Principles of Colorimetry

Quality Control

15. Blood Glucose

16. Glucose Tolerance Test

17. Blood Urea and BUN

18. Serum and Urinary Creatinine

19. Uric Acid

20. Serum Total Proteins and Albumin–Globulin Ratio

21. Cholesterol and Lipid Profile

22. Bilirubin

23. Aminotransferases (AST and ALT)

24. Alkaline and Acid Phosphatases (ALP and ACP)

25. Creatine Kinase and its Isoenzymes

26. Amylase and Lipase

27. Calcium and Phosphorus

28. Serum Electrolytes 171

29. Quantitative Analysis of CSF

30. Electrophoresis

31. Chromatography

32. Spectroscopic Examination of Hemoglobin and its Derivatives

33. Radioimmunoassay and Enzyme-linked Immunosorbant Assay